Electroporation of S-HEK Cells Triggers Action Potentials Dependent on Gap Junction Coupling

Electroporation of S-HEK cells results in multiple action potentials and intricate calcium dynamics, a phenomenon that is contingent upon gap junction coupling. This electrical stimulation method, when applied to these specific cells, induces complex cellular responses. The observed action potentials and the subsequent calcium signaling pathways are directly influenced by the functional state of the gap junctions within the cell population. If gap junctions are functional and allow for intercellular communication, the cellular response to electroporation is amplified and becomes more complex. Conversely, disruptions or absence of gap junction coupling significantly alters or diminishes these observed electrical and calcium dynamics. This highlights the critical role of intercellular communication in mediating cellular reactions to external stimuli like electroporation. Further research into these dynamics could provide insights into cellular electrical excitability and signaling mechanisms, particularly in contexts where gap junction function is paramount.